Experimental Neospora caninum infection in chickens (Gallus gallus domesticus) with oocysts and tachyzoites of two recent isolates reveals resistance to infection. Issue 2 (February 2018)

• Record Type: Journal Article
• Authors: Oliveira, Solange; Aizawa, Juliana; Soares, Herbert Sousa; Chiebao, Daniela Pontes; Castro, Márcio Botelho de; Hora, Aline S.; Lopes, Marcos Gomes; Schares, Gereon; Jenkins, Mark C.; Kwok, Oliver C.H.; Dias, Ricardo Augusto; Gennari, Solange Maria; Dubey, Jitender P.; Pena, Hilda Fátima Jesus
• Is Part Of: International journal for parasitology. Volume 48:Issue 2(2018)
• Page Start: 117
• Page End: 123
• Publication Date: 2018-02
• Subjects: Neosporosis -- Bioassay in dogs -- Chickens -- Intermediate hosts -- Faeces -- PCR; Parasitology -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; Parasitology; Periodicals; Electronic journals; 571.999
• DOI: 10.1016/j.ijpara.2017.07.004
• Languages: English
• ISSNs: 0020-7519

Abstract

Graphical abstract: Highlights: Experimental oral infection of chickens with Neospora caninum oocysts (NC-SP1). Experimental infection of chickens with N. caninum tachyzoites (NcWTDMn1). Neospora caninum was not detected in bird tissues by PCR, qPCR or immunohistochemistry. Dogs that ingested tissues from inoculated birds did not excrete N. caninum oocysts. Chickens are resistant to experimental N. caninum infection. Abstract: The importance of birds in the biological cycle of Neospora caninum is not clear. We report unsuccessful Neospora infection in chickens ( Gallus gallus domesticus ) using two isolates of N. caninum . In experiment #1, 30 White Leghorn chickens were orally inoculated with viable N. caninum oocysts (NC-SP1 isolate, 200 oocysts per bird) via the crop at 21 days of age. Groups of three birds were euthanised at intervals of 7 days (a total of 9 weeks) and one group was challenged with the same oocyst dose at 37 days p.i. and observed for 11 weeks. Blood samples were collected weekly, and sera were tested using IFAT. Chicken tissues were collected for PCR, quantitative PCR and immunohistochemistry. Two dogs approximately 45 days of age were fed with tissues from chickens euthanised at 138 and 159 days p.i. The results indicated that the chickens were resistant to neosporosis as revealed by failure to seroconvert, to detect parasite DNA or N. caninum antigen by immunohistochemistry in inoculated bird tissues, and by no oocyst excretion by the dogs fed avian tissues. Similar results were obtained in experiment #2, in which 34 1-week-old chickens were each s.c. inoculated with 100, 000 tachyzoites of the NcWTDMn1 isolate of N. caninum. The chickens were euthanised on days 7, 15, 22, 28, 36 and 60 p.i. At necropsy, all tissues and serum from each bird were collected. All chickens remained asymptomatic, and N. caninum antigen was not detected by immunohistochemistry. Seven chickens euthanised at day 60 p.i. demonstrated low (1:25 dilution) levels of antibodies by using the Neospora agglutination test. Two 12-week-old dogs fed tissues pooled from 10 inoculated chickens euthanised at day 60 p.i. did not excrete N. caninum oocysts. This investigation indicates that chickens are resistant to experimental infection by N. caninum.