A novel algorithm for segmentation of leukocytes in peripheral blood. (August 2018)

• Record Type: Journal Article
• Authors: Cao, Haichao; Liu, Hong; Song, Enmin
• Is Part Of: Biomedical signal processing and control. Volume 45(2018)
• Page Start: 10
• Page End: 21
• Publication Date: 2018-08
• Subjects: Concave-convex iterative repair method -- Fuzzy divergence -- Interval-valued fuzzy sets -- Leukocyte segmentation -- Stepwise averaging method; Signal processing -- Periodicals; Biomedical engineering -- Periodicals; Signal Processing, Computer-Assisted -- Periodicals; Image Processing, Computer-Assisted -- Periodicals; Biomedical Engineering -- Periodicals; 610.28
• DOI: 10.1016/j.bspc.2018.05.010
• Languages: English
• ISSNs: 1746-8094

Abstract

Highlights: We propose a novel thresholding method and color transformation methods. The G, H and S three single-channel images are considered, which improves the adaptability of the algorithm to various backgrounds. In order to deal with the situation of connected cells, an improved concave-convex iterative repair algorithm is proposed. Abstract: In the detection of anemia, leukemia and other blood diseases, the number and type of leukocytes are essential evaluation parameters. However, the conventional leukocyte counting method is not only quite time-consuming but also error-prone. Consequently, many automation methods are introduced for the diagnosis of medical images. It remains difficult to accurately extract related features and count the number of cells under the variable conditions such as background, staining method, staining degree, light conditions and so on. Therefore, in order to adapt to various complex situations, we consider RGB color space, HSI color space, and the linear combination of G, H and S components, and propose a fast and accurate algorithm for the segmentation of peripheral blood leukocytes in this paper. First, the nucleus of leukocyte was separated by using the stepwise averaging method. Then based on the interval-valued fuzzy sets, the cytoplasm of leukocyte was segmented by minimizing the fuzzy divergence. Next, post-processing was carried out by using the concave-convex iterative repair algorithm and the decision mechanism of candidate mask sets. Experimental results show that the proposed method outperforms the existing non-fuzzy sets methods Among the methods based on fuzzy sets, the interval-valued fuzzy sets perform slightly better than interval-valued intuitionistic fuzzy sets and intuitionistic fuzzy sets.