Ferrocene conjugated oligonucleotide for electrochemical detection of DNA base mismatch. Issue 15 (1st August 2017)

• Record Type: Journal Article
• Authors: Hasegawa, Yusuke; Takada, Tadao; Nakamura, Mitsunobu; Yamana, Kazushige
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 27:Issue 15(2017)
• Page Start: 3555
• Page End: 3557
• Publication Date: 2017-08-01
• Subjects: Ferrocene -- Oligonucleotide -- Conjugate -- Electrochemical detection -- DNA mismatch; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2017.05.049
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: We describe the synthesis, binding, and electrochemical properties of ferrocene-conjugated oligonucleotides (Fc-oligos). The key step for the preparation of Fc-oligos contains the coupling of vinylferrocene to 5-iododeoxyuridine via Heck reaction. The Fc-conjugated deoxyuridine phosphoramidite was used in the Fc-oligonucleotide synthesis. We show that thiol-modified Fc-oligos deposited onto gold electrodes possess potential ability in electrochemical detection of DNA base mismatch.