A novel method for source-specific hemoglobin adducts of nitro-polycyclic aromatic hydrocarbons. Issue 5 (21st March 2018)

• Record Type: Journal Article
• Authors: Wheelock, Kylie; Zhang, Junfeng (Jim); McConnell, Rob; Tang, Deliang; Volk, Heather E.; Wang, Ya; Herbstman, Julie B.; Wang, Shuang; Phillips, David H.; Camann, David; Gong, Jicheng; Perera, Frederica
• Is Part Of: Environmental science. Volume 20:Issue 5(2018)
• Page Start: 780
• Page End: 789
• Publication Date: 2018-03-21
• Subjects: Environmental monitoring -- Periodicals; Biological monitoring -- Periodicals; Environmental chemistry -- Periodicals; 363.7363
• DOI: 10.1039/c7em00522a
• Languages: English
• ISSNs: 2050-7887

Abstract

Abstract : Polycyclic aromatic hydrocarbons (PAH) are ubiquitous air pollutants associated with negative impacts on growth, development and behavior in children. Abstract : Polycyclic aromatic hydrocarbons (PAH) are ubiquitous air pollutants associated with negative impacts on growth, development and behavior in children. Source-specific biological markers of PAH exposure are needed for targeting interventions to protect children. Nitro-derivatives of PAH can act as markers of exposure to diesel exhaust, gasoline exhaust, or general combustion sources. Using a novel HPLC-APCI-MS/MS detection method, we examined four hemoglobin (Hb) adducts of nitro-PAH metabolites and the Hb adduct of a benzo[ a ]pyrene (BaP) metabolite in 22 umbilical cord blood samples. The samples were collected from a birth cohort with comprehensive data on prenatal PAH exposure, including prenatal personal air monitoring and DNA adducts in maternal and umbilical cord blood. Using non-parametric analyses, heat maps, and principal component analysis (PCA), we analyzed the relationship between the five Hb adducts and previous PAH measurements, with each measurement representing a different duration of exposure. We found that Hb adducts derived from several diesel-related nitro-PAHs (2-nitrofluorene and 1-nitropyrene) were significantly correlated ( r = 0.77, p ≤ 0.0001) and grouped together in PCA. Nitro-PAH derived Hb adducts were largely unrelated to previously collected measures of exposure to a number of PAH parent compounds. These measures need to be validated in a larger sample.