Quantitative synthesis of protein–DNA conjugates with 1 : 1 stoichiometry. Issue 54 (19th June 2018)

• Record Type: Journal Article
• Authors: Yan, Xiaowen; Zhang, Hongquan; Wang, Zhixin; Peng, Hanyong; Tao, Jeffrey; Li, Xing-Fang; Chris Le, X.
• Is Part Of: Chemical communications. Volume 54:Issue 54(2018)
• Page Start: 7491
• Page End: 7494
• Publication Date: 2018-06-19
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc03268h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We describe here a binding-facilitated reaction strategy, enabling quantitative conjugation of DNA to native proteins with a desirable 1 : 1 stoichiometry. Abstract : We describe here a binding-facilitated reaction strategy, enabling quantitative conjugation of DNA to native proteins with a desirable 1 : 1 stoichiometry. The technique takes advantage of the iterative affinity interaction and covalent binding processes to achieve complete conjugation. The complete conjugation obviates the need for separation of the protein–DNA conjugates as required by other DNA–protein conjugation methods.