Biofunctionalized mesoporous silica nanospheres for the ultrasensitive chemiluminescence immunoassay of tumor markers. (18th June 2018)

• Record Type: Journal Article
• Authors: Li, Juan; Li, Xinhui; Huang, Ying; Zhong, Yihong; Lan, Qingchun; Wu, Xinyue; Hu, Ruixuan; Zhang, Geshan; Hu, Xiaoya; Yang, Zhanjun
• Is Part Of: New journal of chemistry. Volume 42:Number 14(2018)
• Page Start: 11264
• Page End: 11267
• Publication Date: 2018-06-18
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c8nj02203h
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : Mesoporous silica nanospheres (SiO2 ) are synthesized and biofunctionalized for the development of an ultrasensitive chemiluminescent (CL) immunosensor for tumor markers. Abstract : In this study, mesoporous silica nanospheres (MSNs) are synthesized and biofunctionalized with streptavidin for the development of an ultrasensitive chemiluminescence (CL) immunoassay. The streptavidin-functionalized MSNs have a large specific surface area and exhibit excellent hydrophilicity, which are favourable to effectively capture biotinylated antibodies and retain their bioactivity. We used α-fetoprotein (AFP) as a model analyte, which can be detected in a wide linear range of 0.01–5.0 ng mL −1 with a very low detection limit down to 9 pg mL −1 (S/N = 3). The assay results of clinical human serum samples using the present method are in acceptable agreement with the reference values obtained from the hospital. This biofunctionalized MSN-based CL immunoassay provides a promising approach for early screening of tumor diseases.