Conjugated polyelectrolytes with a label-free aptamer for specific protein photoinactivation. Issue 19 (18th April 2018)

• Record Type: Journal Article
• Authors: Fang, Wei; Liu, Shuwen; Tan, Chunyan; Li, Anqi; Tan, Ying; Jiang, Yuyang
• Is Part Of: Analytical methods. Volume 10:Issue 19(2018)
• Page Start: 2205
• Page End: 2210
• Publication Date: 2018-04-18
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c8ay00272j
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A novel and convenient chromophore-assisted light inactivation strategy is developed for specific and efficient protein inactivation based on conjugated polyelectrolytes and a label-free aptamer. Abstract : Chromophore-assisted light inactivation (CALI) is a significant technique to realize knockdown or loss-of-function of target protein molecules in a spatiotemporally regulated manner. Based on the advantages of CALI, we hybridized PPET3-N2, a well-studied cation conjugated polyelectrolyte (CP), with a label-free DNA containing aptamer via electronic and hydrophobic interactions to produce reactive oxygen species (ROS) under light irradiation, which resulted in specific inactivation of the protein of interest, thrombin. The aptamer introduced into the system formed a complex with thrombin, whereby the aptamer decreased the distance between the PPET3-N2/DNA hybrid and thrombin. This proposed strategy efficiently inactivated thrombin with good specificity and provides a novel and universal approach for controlled inactivation of biomolecules.