Detection of pulmonary surfactant protein A by using an aptamer-based liquid crystal biosensor. Issue 24 (7th June 2018)

• Record Type: Journal Article
• Authors: Wang, Ying; Wang, Bing; Zhang, Qingli; Xiong, Xingliang; Deng, Shixiong
• Is Part Of: Analytical methods. Volume 10:Issue 24(2018)
• Page Start: 2895
• Page End: 2900
• Publication Date: 2018-06-07
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c8ay00326b
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : An aptamer-based liquid crystal biosensor for the detection of pulmonary surfactant protein A has been presented. Abstract : This paper reports a novel detection method for pulmonary surfactant protein A (SP-A) using an aptamer-based liquid crystal (LC) biosensor. The LC molecules are supported on the surface of glass slides modified with an appropriate amount of SP-A aptamers, causing a homeotropic orientation of LCs. The specific interaction between the aptamer and SP-A induces great changes of the topology on the surface. The changes will disrupt the homeotropic orientation of LCs, resulting in an optical appearance transition of the polarized image. Therefore, SP-A can be easily recognized. The results can be quantitatively determined through image analysis. The limit of detection is only 5 nM. The presented strategy provides a simple aptamer-based sensing platform for biomolecule recognition, owing to its characteristics of being label-free, low-cost and highly specific, and having no requirement of instrumental read-out.