Fluorescent DNA‐Templated Silver Nanoclusters from Silver(I)‐Mediated Base Pairs. Issue 33 (17th May 2018)

• Record Type: Journal Article
• Authors: Léon, J. Christian; González‐Abradelo, Darío; Strassert, Cristian A.; Müller, Jens
• Is Part Of: Chemistry. Volume 24:Issue 33(2018)
• Page Start: 8320
• Page End: 8324
• Publication Date: 2018-05-17
• Subjects: cytosine -- DNA -- imidazole -- metal-mediated base pairs -- silver nanoclusters; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201801858
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: Silver nanoclusters (AgNCs) stabilized by double‐stranded DNA (dsDNA) are of special interest because the duplex structures provide rigidity and chirality that can be transferred to the metal clusters. This work reports fluorescent AgNCs obtained from dsDNA templates containing artificial ligand‐derived nucleobases. They are compared with fluorescent AgNCs obtained from DNA templates containing C:C mismatches (C, cytosine). Towards this end, the new metal‐mediated Im−Ag I −C base pair composed of imidazole (Im) and cytosine was introduced into dsDNA and reduced to form AgNCs. The clusters were characterized by UV/Vis, fluorescence and circular dichroism spectroscopy. The AgNCs form chiral aggregates with dsDNA. Their optical properties are highly sequence‐dependent. As a result, a simple method to detect a cytosine insertion into a run of cytosine residues in a duplex is proposed using the human CDH1 gene as an example. Abstract : Silver dream machine : A new concept for the generation of DNA‐templated fluorescent silver nanoclusters is introduced, applying double‐stranded DNA templates bearing site‐specifically introduced silver(I) ions.