Distinct Roles of NMDAR and mGluR5 in Light Exposure Reversal of Feedforward Synaptic Strength in V1 of Juvenile Mice after Binocular Vision Deprivation. (1st August 2018)

• Record Type: Journal Article
• Authors: Tie, Xiaoxiu; Li, Shuo; Feng, Yilin; Lai, Biqin; Liu, Sheng; Jiang, Bin
• Is Part Of: Neuroscience. Volume 384(2018)
• Page Start: 131
• Page End: 138
• Publication Date: 2018-08-01
• Subjects: CPP carboxypiperazin-4-yl-propyl-1-phosphonic acid -- HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid -- LTD long-term depression -- LTP long-term potentiation -- MPEP 2-methyl-6-(phenylethynyl) pyridine hydrochloride -- NMDAR NMDA receptor; visual cortex -- feedforward excitatory synaptic strength -- dark rearing -- dark exposure -- light exposure; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2018.05.034
• Languages: English
• ISSNs: 0306-4522

Abstract

Graphical abstract: Highlights: Light exposure reverses feedforward synaptic strength in juvenile mouse visual cortex, which was reduced by DR/DE. Feedforward synaptic strength is negatively correlated with NMDAR LTP induction in juvenile mouse visual cortex. NMDAR and mGluR5 play distinct roles in experience-dependent feedforward synaptic strengthening. Abstract: In the visual cortex, sensory deprivation causes global augmentation of the amplitude of AMPA receptor-mediated miniature EPSCs in layer 2/3 pyramidal cells and enhancement of NMDA receptor-dependent long-term potentiation (LTP) in cells activated in layer 4, effects that are both rapidly reversed by light exposure. Layer 2/3 pyramidal cells receive both feedforward input from layer 4 and intra-cortical lateral input from the same layer, LTP is mainly induced by the former input. Whether feedforward excitatory synaptic strength is affected by visual deprivation and light exposure, how this synaptic strength correlates with the magnitude of LTP in this pathway, and the underlying mechanism have not been explored. Here, we showed that in juvenile mice, both dark rearing and dark exposure reduced the feedforward excitatory synaptic strength, and the effects can be reversed completely by 10–12 h and 6–8 h light exposure, respectively. However, inhibition of NMDA receptors by CPP or mGluR5 by MPEP, prevented the effect of light exposure on the mice reared in the dark from birth, while only inhibition of NMDAR prevented the effect of light exposure on dark-exposed mice. These results suggested that the activation of both NMDAR and mGluR5 are essential in the light exposure reversal of feedforward excitatory synaptic strength in the dark reared mice from birth; while in the dark exposed mice, only activation of NMDAR is required.