Reconstituting donor T cells increase their biomass following hematopoietic stem cell transplantation. Issue 11 (28th March 2018)

• Record Type: Journal Article
• Authors: Leslie, Kevin A.; Rasheed, Mahmood; Sabo, Roy T.; Roberts, Catherine C.; Toor, Amir A.; Reed, Jason
• Is Part Of: Analyst. Volume 143:Issue 11(2018)
• Page Start: 2479
• Page End: 2485
• Publication Date: 2018-03-28
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an00148k
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : In this study, we used a rapid, highly-sensitive, single-cell biomass measurement method, Live Cell Interferometry (LCI), to measure biomass in populations of CD3 + T cells isolated from hematopoietic stem cell transplant (SCT) patients at various times pre- and post-transplant (days 0–100). Abstract : In this study, we used a rapid, highly-sensitive, single-cell biomass measurement method, Live Cell Interferometry (LCI), to measure biomass in populations of CD3 + T cells isolated from hematopoietic stem cell transplant (SCT) patients at various times pre- and post-transplant (days 0–100). CD3 + T cell 'mass spectra' were obtained from five autologous and 20 allogenic transplant recipients. We found a pronounced rise in median T cell biomass (+25%; p <0.001) shortly after transplant (day 14), which moderated by day 60. Further, the inter-patient and intra-patient cell masses were most variable at days 14 and 30 post-transplant. T cell biomass trends were similar in both autologous and allogenic transplant recipients. These data suggest that T cell biomass changes are associated with immune reconstitution occurring in the first few weeks post-transplant. To our knowledge, this is the first time single-cell biomass measurements have been studied in human clinical trials. With refinement, these data may prove useful in guiding the withdrawal of immunosuppression following SCT, reducing the likelihood of Graft-Versus-Host Disease or cancer relapse occurring.