Sticky-flares for in situ monitoring of human telomerase RNA in living cells. Issue 19 (9th May 2018)

• Record Type: Journal Article
• Authors: Wu, Qilong; Liu, Zhengjie; Su, Lei; Han, Guangmei; Liu, Renyong; Zhao, Jun; Zhao, Tingting; Jiang, Changlong; Zhang, Zhongping
• Is Part Of: Nanoscale. Volume 10:Issue 19(2018)
• Page Start: 9386
• Page End: 9392
• Publication Date: 2018-05-09
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c8nr01260a
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : A novel DNA-conjugated AuNP probe termed sticky-flares has been designed for the in situ detection of intracellular human telomerase RNA. Abstract : Human telomerase RNA (hTR), a template of telomerase for telomeric repeat synthesis, was used to reflect the telomerase activity and act as a potential target of antitumor therapy. Here, we report a novel DNA-conjugated AuNP probe termed sticky-flares for the in situ detection of intracellular human telomerase RNA. The sticky-flares probe is capable of entering living cells directly without any auxiliary and recognizing the binding domain of human telomerase RNA. On recognition, the fluorophore-modified recognition flares can specifically bind to the target, separate from the sticky-flares and act as a fluorescent reporter to quantify and dynamically profile human telomerase RNA in living cells. We envision that the sticky-flares probe would be a valuable platform to investigate the function and regulation of hTR in antitumor therapy and hTR-related drug invention.