The genomic and transcriptomic analyses of serine proteases and their homologs in an endoparasitoid, Pteromalus puparum. (December 2017)

• Record Type: Journal Article
• Authors: Yang, Lei; Lin, Zhe; Fang, Qi; Wang, Jiale; Yan, Zhichao; Zou, Zhen; Song, Qisheng; Ye, Gongyin
• Is Part Of: Developmental and comparative immunology. Volume 77(2017)
• Page Start: 56
• Page End: 68
• Publication Date: 2017-12
• Subjects: Pteromalus puparum -- Serine protease -- Serine protease homolog -- Expression profile -- Venom gland; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2017.07.014
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: In insects, serine proteases (SPs) and serine protease homologs (SPHs) constitute a large family of proteins involved in multiple physiological processes such as digestion, development, and immunity. Here we identified 145 SPs and 38 SPHs in the genome of an endoparasitoid, Pteromalus puparum . Gene duplication and tandem repeats were observed in this large SPs/SPHs family. We then analyzed the expression profiles of SP/SPH genes in response to different microbial infections (Gram-positive bacterium Micrococcus luteus, Gram-negative bacterium Escherichia coli, and entomopathogenic fungus Beauveria bassiana ), as well as in different developmental stages and tissues. Some SPs/SPHs also displayed distinct expression patterns in venom gland, suggesting their specific physiological functions as venom proteins. Our finding lays groundwork for further research of SPs and SPHs expressed in the venom glands. Highlights: One hundred and eighty-three serine proteases (SPs) and serine protease homologs (SPHs) were identified. Detailed information of SPs and SPHs was revealed by genome scale analysis. Tandem repeats of SPs and SPHs with similar genomic structures indicated gene duplication events happened. Six SPs and two SPHs were predicted as venom proteins.