Development of an ON/OFF switchable fluorescent probe targeting His tag fused proteins in living cells. Issue 15 (1st August 2017)

• Record Type: Journal Article
• Authors: Okitsu, Koyo; Misawa, Takashi; Shoda, Takuji; Kurihara, Masaaki; Demizu, Yosuke
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 27:Issue 15(2017)
• Page Start: 3417
• Page End: 3422
• Publication Date: 2017-08-01
• Subjects: Cell-penetrating peptide -- Maleimide -- ON/OFF switch -- His tag fused protein; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2017.05.087
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: The fluorescent labeling of target proteins is useful for analyzing their functions and localization in cells, and several fluorescent probes have been developed. However, the fusion of tags such as green fluorescent protein (GFP) to target proteins occasionally affects their functions and/or localization in living cells. Therefore, an imaging method that uses short peptide tags such as hexa-histidine (the His tag) has been attracting increasing attention. Few studies have investigated ON/OFF switchable fluorescent probes for intracellular His-tagged proteins. We herein developed a novel ON/OFF switchable probe for imaging targeted intracellular proteins fused with a CH6 tag, which is composed of one cysteine residue and six histidine residues.