Autophagy induced by infectious hematopoietic necrosis virus inhibits intracellular viral replication and extracellular viral yields in epithelioma papulosum cyprini cell line. (December 2017)

• Record Type: Journal Article
• Authors: Zhao, Jing-Zhuang; Xu, Li-Ming; Liu, Miao; Zhang, Zhen-yu; Yin, Jia-Sheng; Liu, Hong-Bai; Lu, Tong-Yan
• Is Part Of: Developmental and comparative immunology. Volume 77(2017)
• Page Start: 88
• Page End: 94
• Publication Date: 2017-12
• Subjects: Infectious hematopoietic necrosis virus -- Autophagy -- LC3 -- Antiviral response; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2017.07.025
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Infectious hematopoietic necrosis virus (IHNV) is a common pathogen that causes severe disease in the salmonid aquaculture industry. Recent work demonstrated that autophagy plays an important role in pathogen invasion by activating innate and adaptive immunity. This study investigated the relationship between IHNV and autophagy in epithelioma papulosum cyprini cells. The electron microscopy results show that IHNV infection can induce typical autophagosomes which are representative structures of autophagy activation. The punctate accumulation of green fluorescence-tagged microtubule-associate protein 1 light chain 3 (LC3) and the protein conversion from LC3-I to LC3-II were respectively confirmed by confocal fluorescence microscopy and western blotting. Furthermore, the effects of autophagy on IHNV replication were also clarified by altering the autophagy pathway. The results showed that rapamycin induced autophagy can inhibit both intracellular viral replication and extracellular viral yields, while autophagy inhibitor produced the opposite results. These findings demonstrated that autophagy plays an antiviral role during IHNV infection. Highlights: IHNV infection induced autophagy in EPC cell line. Induction of autophagy inhibited IHNV replication and extracellular viral yields. Autophagy plays an antiviral role during IHNV infection in EPC cells.