An improved clearing and mounting solution to replace chloral hydrate in microscopic applications1. (7th May 2013)

• Record Type: Journal Article
• Authors: Villani, Thomas S.; Koroch, Adolfina R.; Simon, James E.
• Is Part Of: Applications in plant sciences. Volume 1:Number 5(2013)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2013-05-07
• Subjects: acidified chloral hydrate -- botanical quality control -- clearing -- histology -- microscopy -- Visikol; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.3732/apps.1300016
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the study: This study presents Visikol™, a new proprietary formulation that can be used as an efficient replacement for chloral hydrate as a clearing agent for microscopic examination. In the United States, chloral hydrate is regulated and therefore difficult to acquire. Methods and Results: Fresh and dry samples of the following plants: ginger ( Zingiber officinale ), maté ( Ilex paraguariensis ), lime basil ( Ocimum americanum ), oregano ( Origanum vulgare ), and mouse‐ear cress ( Arabidopsis thaliana ), were cleared using Visikol or chloral hydrate solution and compared using a light microscope. Conclusions: This new method can be used successfully to clear specimens, allowing identification of diagnostic characteristics for the identification of plant materials. Visikol is as effective as chloral hydrate in providing clarity and resolution of all tissues examined. Tissues become transparent, allowing observation of deeper layers of cells and making it effective in research, botanical and quality control, and for educational applications.