Temperature-dependent development of Asian citrus psyllid on various hosts, and mortality by two strains of Isaria. (June 2018)

• Record Type: Journal Article
• Authors: Qasim, Muhammad; Lin, Yongwen; Dash, Chandra Kanta; Bamisile, Bamisope Steve; Ravindran, Keppanan; Islam, Saif Ul; Ali, Habib; Wang, Fangfei; Wang, Liande
• Is Part Of: Microbial pathogenesis. Volume 119(2018)
• Page Start: 109
• Page End: 118
• Publication Date: 2018-06
• Subjects: Developmental time -- Isaria fumorosea -- Isaria javanica -- Candidatus Liberibacter asiaticus -- HLB-diseased plants -- Phylogenetic analyses; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.04.019
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is a devastating pest of Citrus spp. The aim of present study was to investigate the development and mortality of ACP on citrus ( Citrus sinensis ) (healthy and Huánglóngbìng- (HLB) diseased) and jasmine ( Murraya paniculata ) plants at various temperatures. Two new Isaria strains were collected from citrus orchards of Fuzhou (China), and HLB-diseased plants were verified by running PCR for 16S gene of Candidatus Liberibacter asiaticus (CLas). Development observations were recorded for egg, nymph and adult stages on all plants and three different temperatures (20, 25 and 30 °C) whereas mortality observations were recorded for the nymph (fifth instar) and adults on all plants at 25 °C. Field collected Isaria strains were belonged to previously reported Chinese strains under Maximum Parsimony (MP) and Maximum Likelihood methods, as well as, CLas isolates were belonged to previously reported Chinese isolates under MP and Neighbor-Joining methods. The fastest development and mortality was observed on HLB-diseased plants whereas longest time was taken by development and mortality completion on jasmine plants at all temperatures. The fastest developmental times of egg, nymph (first to fourth and fifth instar) and adult stages were ranged from 3.02 to 3.72 d, to 7.63–9.3 d, 5.35–5.65 d and 24.46–28.47 d on HLB-diseased plants at 30-20 °C, respectively. On the other hand, I. javanica caused the fastest mortality of nymphs and adults (32.21 ± 4.47% and 19.33 ± 4.51%) on HLB-diseased plants with the concentration of 1 × 10 8 conidia.mL −1 after 3 d and 7 d, respectively. It is concluded that there is a need for extensive molecular work to understand the extra-development and mortality of ACP on diseased plants, because, CLas bacterium can be supportive to uptake more sap from plant phloem. Highlights: ACP (Asian citrus psyllid) development was studied on various host plants at different temperatures. Development of all stages was rapid on HLB-(Huanglongbing) diseased plants, and these plants were verified through 16S gene. Two new Isaria strains were identified through ITS gene, and their potential was assessed against nymphs and adults of ACP. Nymphs were less susceptible to Entomopathogenic fungus, as compared to adults. ACP (nymph and adult) mortality was higher on HLB-diseased plants, as compared to other plants.