The establishment of methods for free PAR generation and PAR reader detection. (June 2018)
- Record Type:
- Journal Article
- Title:
- The establishment of methods for free PAR generation and PAR reader detection. (June 2018)
- Main Title:
- The establishment of methods for free PAR generation and PAR reader detection
- Authors:
- Ke, Yueshuang
Wang, Ke
Xu, Hui
Wang, Chenxin
Zhang, Jing
Zeng, Xianlu
Wang, Ruoxi
Boldogh, Istvan
Ba, Xueqing - Abstract:
- Abstract: Poly (ADP-ribose) polymerase 1 (PARP1) is a DNA damage sensor that catalyzes the poly (ADP-ribose) (PAR) onto a variety of target proteins, such as histones, DSB repair factors and PARP1 itself under consumption of NAD + . Besides, PARP1 can affect a variety of proteins in noncovalent modification manner to carry out specific cellular functions. Here, we established a method to generate non-radiolabeled free PAR by PARG moderately cleaving PAR from autoPARylated PARP1, and utilized dot-blot assay to determine the interaction between free PAR and interested proteins. The methods to generate free PAR and detect the noncovalent interactions between proteins and free PAR are nonradioactive and convenient, which will facilitate the studies to explore the significance of PAR reading in various biological processes. Highlights: Set up of a rapid, convenient and nonradioactive method to generate and detect free PAR. RNA binding protein HuR, which can be covalently PARylated by PARP1, is not a PAR reader. This method will facilitate the studies to explore the significance of PAR reading in various biological processes.
- Is Part Of:
- Molecular and cellular probes. Volume 39(2018)
- Journal:
- Molecular and cellular probes
- Issue:
- Volume 39(2018)
- Issue Display:
- Volume 39, Issue 2018 (2018)
- Year:
- 2018
- Volume:
- 39
- Issue:
- 2018
- Issue Sort Value:
- 2018-0039-2018-0000
- Page Start:
- 57
- Page End:
- 60
- Publication Date:
- 2018-06
- Subjects:
- PARP1 -- Free PAR -- PARG -- Dot-blot assay -- Noncovalent
Molecular probes -- Diagnostic use -- Periodicals
Pathology, Cellular -- Technique -- Periodicals
Cell Biology -- Periodicals
Molecular Biology -- Periodicals
Sondes moléculaires -- Utilisation diagnostique -- Périodiques
Cytopathologie -- Technique -- Périodiques
572 - Journal URLs:
- http://www.sciencedirect.com/science/journal/08908508 ↗
http://firstsearch.oclc.org ↗
http://firstsearch.oclc.org/journal=0890-8508;screen=info;ECOIP ↗
http://www.elsevier.com/journals ↗ - DOI:
- 10.1016/j.mcp.2018.04.002 ↗
- Languages:
- English
- ISSNs:
- 0890-8508
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 5900.761000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 6843.xml