Characterization of a fluorescent hydrogel synthesized using chitosan, polyvinyl alcohol and 9-anthraldehyde for the selective detection and discrimination of trace Fe3+ and Fe2+ in water for live-cell imaging. (1st August 2018)

• Record Type: Journal Article
• Authors: Maity, Santu; Parshi, Nira; Prodhan, Chandraday; Chaudhuri, Keya; Ganguly, Jhuma
• Is Part Of: Carbohydrate polymers. Volume 193(2018)
• Page Start: 119
• Page End: 128
• Publication Date: 2018-08-01
• Subjects: Biopolymer -- Fluorescent hydrogel -- Selective detection -- Fe3+/Fe2+ discrimination -- Live-cell imaging; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.03.073
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: A chitosan based hydrogel with superior fluorescence properties has been synthesized. The emission intensity of the hydrogel enhanced remarkably for Fe 3+ and strongly quenched for Fe 2+ . The fluorometric detection limits for Fe 3+ and Fe 2+ were found to be 0.124 and 0.138 nM respectively. The probe is also promising as a selective sensor of Fe 3+ and Fe 2+ in the fluorescence imaging of living cells. Abstract: A three-dimensional fluorescent hydrogel based on chitosan, polyvinyl alcohol and 9-anthraldehyde (ChPA) has been successfully designed and synthesized for the selective detection and discrimination of Fe 3+ and Fe 2+ in aqueous environment. The unique characteristics of ChPA has been confirmed by the Fourier-transform infrared spectroscopy (FTIR), rheological measurement, scanning electron microscopy (SEM), thermogravimetry and differential thermogravimetry (TG-DTG), ultraviolet–visible spectroscopy (UV–vis), fluorescence studies, transmission electron microscopy (TEM), energy dispersive x-ray spectroscopy (EDX), x-ray diffraction (XRD) and dynamic light scattering (DLS). The emission intensity at 516 nm of the hydrogel has been enhanced remarkably with the addition of Fe 3+ due to the inhibition of the photoinduced electron transfer (PET) process. However, it gets strongly quenched in the case of Fe 2+ owing to chelation enhanced quenching (CHEQ). The probe (ChPA) causes no significant change in the fluorescence and becomes highly specific and sensitive towards Fe 3+ and Fe 2+ compared to other interfering heavy and transition metal ions (HTM). The detection limits of the sensor for the Fe 3+ and Fe 2+ are 0.124 nM and 0.138 nM, respectively. The probe is also promising as a selective sensor for the Fe 3+ and Fe 2+ in the fluorescence imaging of living cells. Thus, such a probe opens up new opportunities to improve the chitosan based fluorescent chemosensor having biocompatibility, biodegradability, sufficient thermal stability and stability in a wide pH range.