Synthesis of chitin-glucan-aldehyde-quercetin conjugate and evaluation of anticancer and antioxidant activities. (1st August 2018)

• Record Type: Journal Article
• Authors: Singh, Anu; Dutta, P.K.; Kumar, Hridyesh; Kureel, Amit Kumar; Rai, Ambak Kumar
• Is Part Of: Carbohydrate polymers. Volume 193(2018)
• Page Start: 99
• Page End: 107
• Publication Date: 2018-08-01
• Subjects: Chitin-glucan complex -- Quercetin -- Chitin-glucan-aldehyde-quercetin conjugate -- Antioxidant properties -- Anticancer properties; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.03.092
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Antioxidant and anticancer properties containing conjugate have been developed. Chitin-glucan aldehyde and quercetin linked via condensation process. The resulting conjugate material was better than anticancer and antioxidant treatment. Safe, non-toxic, polymeric conjugate for biomedical application. Abstract: In the present study, we have synthesized chitin-glucan-aldehyde-quercetin (chi-glu-ald-que) conjugate via condensation reaction. Synthesis of chitin-glucan-aldehyde (chi-glu-ald) complex was facilitated by the oxidation of chitin-glucan (chi-glu) complex. Formation of conjugate was confirmed by Proton nuclear magnetic resonance spectroscopy ( 1 H NMR) and Fourier-transform infrared spectroscopy (FT-IR). Morphological studies showed that after grafting of quercetin, several changes on surface were depicted and a more crystalline nature was observed. The chi-glu-ald-que conjugate displayed strong antioxidant activity. It showed 69% of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, DPPH* scavenging activity at 1 mg/mL and 72% of 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation, ABTS* + scavenging activity at 1 mg/mL concentration, which are much higher than that of chi-glu complex. The anticancer activity of chi-glu-ald-que conjugate was performed in Macrophage cancer cell lines (J774) and biocompatibility was performed in Peripheral blood mononuclear cells (PBMCs). The chi-glu-ald-que conjugate showed excellent cytotoxicity against J774 cell lines but no cytotoxicity towards PBMCs.