Upregulation of VEGFR1 in a rat model of esophagogastric anastomotic healing. Issue 3 (4th May 2018)

• Record Type: Journal Article
• Authors: Landes, Laura Christina; Drescher, Daniel; Tagkalos, Evangelos; Grimminger, Peter P.; Thieme, René; Jansen-Winkeln, Boris; Lang, Hauke; Gockel, Ines
• Is Part Of: Acta chirurgica belgica. Volume 118:Issue 3(2018)
• Page Start: 161
• Page End: 166
• Publication Date: 2018-05-04
• Subjects: Gastrointestinal anastomosis -- growth factor receptors -- anastomotic leakage -- wound healing -- experimental surgery -- stomach; Surgery -- Periodicals; General Surgery; Chirurgie -- Périodiques; Surgery; Chirurgie (geneeskunde); Periodicals; Periodicals; 617.005
• DOI: 10.1080/00015458.2017.1394673
• Languages: English
• ISSNs: 0001-5458

Abstract

Abstract: Introduction: Anastomotic leakage after gastrointestinal surgery is a significant cause of morbidity and mortality. Esophagogastric and colorectal anastomoses are vulnerable to leakage. Extended knowledge of growth factors and their receptors is needed to understand anatomic healing. Methods: The expression pattern of vascular growth factor receptor (VEGFR1-3), epidermal growth factor receptor (EGFR), platelet-derived growth factor receptor (PDGFRα/β) and keratinocyte growth factor receptor (KGFR) were analyzed by semiquantitative-PCR in the rat intestinal tract and in esophagogastric anastomosis 5d after surgery. Results: VEGFR1, VEGFR2, EGFR, KGFR and PDGFRα expression was observed throughout the intestinal tract including esophagus, stomach, small bowl and colon. VEGFR3 was not found in gastric samples and PDGFRβ expression was not detected in the small bowl. Semiquantitative analyses of the VEGFR1, PDGFRα and EGFR expression in esophagogastric anastomotic tissues revealed a 2-fold upregulation of the VEGFR1 in gastric samples, while no change was observed in the esophageal anastomotic side. Conclusion: Our results revealed a distinct expression pattern of the investigated growth factor receptors in rat intestinal tract. Showing higher expression levels of growth factor receptors at the gastric anastomotic tissue at the fifth postoperative day suggests a different contribution of the gastric and the esophageal side to the anastomotic healing.