Effects of molsidomine on retinal ischemia/reperfusion injury in rabbits. (3rd April 2018)

• Record Type: Journal Article
• Authors: Polat, N; Ozer, MA; Parlakpinar, H; Vardi, N; Aksungur, Z; Ozhan, O; Yildiz, A; Turkoz, Y
• Is Part Of: Biotechnic & histochemistry. Volume 93:Number 3(2018)
• Page Start: 188
• Page End: 197
• Publication Date: 2018-04-03
• Subjects: apoptosis -- ischemia/reperfusion -- molsidomine -- oxidative stress -- rabbit -- retina; Histochemistry -- Periodicals; Stains and staining (Microscopy) -- Periodicals; Biotechnology -- Periodicals; 572
• DOI: 10.1080/10520295.2017.1406616
• Languages: English
• ISSNs: 1052-0295

Abstract

Abstract: We investigated the effect of molsidomine (MOL) on ischemia/reperfusion (I/R) injury. Rabbits were assigned to four groups: group 1, sham; group 2, I/R; group 3, MOL treatment for 4 days after I/R; group 4, MOL treatment for 1 day before I/R and 3 days after I/R. Retinal I/R was produced by elevating the intraocular pressure to 150 mm Hg for 60 min. Seven days after I/R, the eyes were enucleated. Retinal changes were examined using histochemistry. The levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) also were measured. We found a significant increase in the thickness of the outer nuclear layer of group 3 compared to the other groups. In groups 3 and 4, caspase-3 stained cells in the ganglion cell layer were decreased compared to group 2. We found a significant increase in caspase-3 stained cells in the inner nuclear layer (INL) of group 2 compared to the other groups. We found a significant increase in caspase-3 stained cells in group 3 compared to group 4 in the INL. The MDA level in group 2 was significantly higher than group 1 and MOL significantly decreased MDA levels in groups 3 and 4. We found that MOL protected the retina from I/R injury by enhancing antioxidative effects and inhibiting apoptosis of retinal cells.