Expression of the alpha toxin of Clostridium perfringens in Lactobacillus casei genome and evaluation of its immune effects in mice. (May 2018)

• Record Type: Journal Article
• Authors: Song, Baifen; Cui, Hongyu; Ju, Longzhu; Song, Li; Tang, Lijie; Li, Yijing
• Is Part Of: Microbial pathogenesis. Volume 118(2018)
• Page Start: 1
• Page End: 8
• Publication Date: 2018-05
• Subjects: Alpha toxin -- Clostridium perfringens -- Immune -- Lactobacillus casei; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.03.010
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: We previously developed a stable and marker-free Lactobacillus casei strain (PPαT Δ upp ) that contained a chromosomally integrated expression cassette (PPαT) that enabled the surface expression of the Clostridium perfringens alpha toxin. To measure immune responses against the alpha toxin, specific-pathogen-free BALB/c mice were inoculated with L. casei PPαT Δ upp by oral gavage. Then, specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies and cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FCM). The results showed that alpha toxin-specific IgA and IgG antibodies and cytokines were markedly increased following immunization. Natural alpha toxin challenge and neutralization tests were performed. The results showed that immunized mice can fully resist 1.5 minimum lethal doses of toxin. These results indicated that the immunized mice can produce not only humoral immunity, but also cellular immunity. These results provide a new pathway for the development of a safe, effective, and food-grade vaccine. Highlights: Lactic acid bacteria (LAB) offer several advantages over current systemic vaccination routes. Integrating antigen-encoding genes into the chromosome is expected to eliminate selection requirements and provide genetic stability. Chromosomal integrated L . casei were immunized to mice. The immunized mice can produce not only humoral immunity, but also cellular immunity. These provide a new pathway for the development of a safe, effective, and food-grade vaccine.