Thyroxin releasing chitosan/collagen based smart hydrogels to stimulate neovascularization. (5th November 2017)

• Record Type: Journal Article
• Authors: Aleem, Abdur Raheem; Shahzadi, Lubna; Alvi, Farah; Khan, Ather Farooq; Chaudhry, Aqif Anwar; ur Rehman, Ihtesham; Yar, Muhammad
• Is Part Of: Materials & design. Volume 133(2017)
• Page Start: 416
• Page End: 425
• Publication Date: 2017-11-05
• Subjects: Thyroxine -- CAM-assay -- Chitosan -- Collagen -- Biodegradable -- Tissue engineering -- Angiogenesis; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2017.07.053
• Languages: English
• ISSNs: 0264-1275

Abstract

Abstract: The development of new biomaterials with tailored properties is highly desired in tissue engineering field. The neovascularization is essential part of tissue regeneration which provides food and nutrients to cells. There is a real need for proangiogenic biomaterials to assist wound healing. The ideal dressing should be inexpensive and achieve rapid healing with minimal inconvenience to the patient. In this paper, new porous thyroxin containing pro-angiogenic hydrogels were generated via freeze gelation protocol. The chemical structural analysis of the synthesized hydrogels was investigated by Fourier Transform Infrared (FTIR) spectroscopy. The morphology and pore dimensions were studied by scanning electron microscopy (SEM). In swelling studies, 10 μg thyroxine loaded hydrogel (TLH-10) showed greater degree of swelling as compared to 1 μg loaded thyroxine material (TLH-1) and control. The degradation studies were tested in three different media, i.e. phosphate buffer saline (PBS), lysozyme and hydrogen peroxide and relatively higher degradation was seen in hydrogen peroxide. The synthesized materials were implanted on the chick chorioallantoic membrane to investigate their angiogenic potential. The TLH-1 hydrogel stimulated angiogenesis greater than the TLH-10; in this case blood vessels were attached and very much grown into the scaffold. Graphical abstract: Highlights: New porous thyroxin containing pro-angiogenic hydrogels were generated via freeze gelation protocol. The synthesized hydrogels showed good level of in vitro degradation. Chorioallantoic membrane assay was used to investigate angiogenesis and tissue growth into hydrogels. The 1 μg thyroxin containing hydrogel showed highest angiogenesis.