Dopamine D1 receptor‐mediated upregulation of BKCa currents modifies Müller cell gliosis in a rat chronic ocular hypertension model. Issue 7 (6th March 2018)

• Record Type: Journal Article
• Authors: Wu, Hang‐Jing; Li, Xue‐Yan; Qian, Wen‐Jing; Li, Qian; Wang, Shu‐Yue; Ji, Min; Ma, Yuan‐Yuan; Gao, Feng; Sun, Xing‐Huai; Wang, Xin; Miao, Yanying; Yang, Xiong‐Li; Wang, Zhongfeng
• Is Part Of: Glia. Volume 66:Issue 7(2018)
• Page Start: 1507
• Page End: 1519
• Publication Date: 2018-03-06
• Subjects: dopamine D1 receptor -- glaucoma -- gliosis -- large‐conductance Ca2+‐activated K+ channel -- Müller cells; Neuroglia -- Periodicals; Neurology -- Periodicals; 611.0188
• DOI: 10.1002/glia.23321
• Languages: English
• ISSNs: 0894-1491

Abstract

Abstract: Müller cell gliosis is a common response in many retinal pathological conditions. We previously demonstrated that downregulation of Kir channels contributes to Müller cell gliosis in a rat chronic ocular hypertension (COH) model. Here, the possible involvement of outward K + currents in Müller cell gliosis was investigated. Outward K + current densities in Müller cells isolated from COH rats, as compared with those in normal rats, showed a significant increase, which was mainly contributed by large‐conductance Ca 2+ ‐activated K + (BKCa ) channels. The involvement of BKCa channels in Müller cell gliosis is suggested by the fact that glial fibrillary acidic protein (GFAP) levels were augmented in COH retinas when these channels were suppressed by intravitreal injections of iberiotoxin. In COH retinas an increase in dopamine (DA) D1 receptor (D1R) expression in Müller cells was revealed by both immunohistochemistry and Western blotting. Moreover, protein levels of tyrosine hydroxylase were also increased, and consistent to this, retinal DA contents were elevated. SKF81297, a selective D1R agonist, enhanced BKCa currents of normal Müller cells through intracellular cAMP‐PKA signaling pathway. Furthermore, GFAP levels were increased by the D1R antagonist SCH23390 injected intravitreally through eliminating the BKCa current upregulation in COH retinas, but partially reduced by SKF81297. All these results strongly suggest that the DA‐D1R system may be activated to a stronger extent in COH rat retinas, thus increasing BKCa currents of Müller cells. The upregulation of BKCa channels may antagonize the Kir channel inhibition‐induced depolarization of Müller cells, thereby attenuating the gliosis of these cells. Main Points: BKCa currents are significantly upregulated in Müller cells in COH retinas. Upregulated BKCa currents attenuate Müller cells gliosis in COH retinas. Activated dopamine/D1 receptor system mediates upregulation of BKCa currents.