Biofunctionalized Plants as Diverse Biomaterials for Human Cell Culture. Issue 8 (20th March 2017)

• Record Type: Journal Article
• Authors: Fontana, Gianluca; Gershlak, Joshua; Adamski, Michal; Lee, Jae‐Sung; Matsumoto, Shion; Le, Hau D.; Binder, Bernard; Wirth, John; Gaudette, Glenn; Murphy, William L.
• Is Part Of: Advanced healthcare materials. Volume 6:Issue 8(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-03-20
• Subjects: biofunctionalization -- biomaterials -- cell expansion -- decellularized scaffolds -- plants; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201601225
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract : The commercial success of tissue engineering products requires efficacy, cost effectiveness, and the possibility of scaleup. Advances in tissue engineering require increased sophistication in the design of biomaterials, often challenging the current manufacturing techniques. Interestingly, several of the properties that are desirable for biomaterial design are embodied in the structure and function of plants. This study demonstrates that decellularized plant tissues can be used as adaptable scaffolds for culture of human cells. With simple biofunctionalization technique, it is possible to enable adhesion of human cells on a diverse set of plant tissues. The elevated hydrophilicity and excellent water transport abilities of plant tissues allow cell expansion over prolonged periods of culture. Moreover, cells are able to conform to the microstructure of the plant frameworks, resulting in cell alignment and pattern registration. In conclusion, the current study shows that it is feasible to use plant tissues as an alternative feedstock of scaffolds for mammalian cells. Abstract : Decellularization and biofunctionalization of plant tissues for expansion of human cells . The elevated hydrophilicity and excellent water transport abilities of plants can be exploited by using plants as a source for biomaterials. Plants can be decellularized and biofunctionalized with RGD–dopamine conjugates or biomineralized to enable effective expansion of human cells.