Identification of VIM‐2 metallo‐β‐lactamase‐producing Pseudomonas aeruginosa isolated from dogs with pyoderma and otitis in Korea. Issue 3 (25th March 2018)

• Record Type: Journal Article
• Authors: Hyun, Jae‐Eun; Chung, Tae‐Ho; Hwang, Cheol‐Yong
• Is Part Of: Veterinary dermatology. Volume 29:Issue 3(2018)
• Page Start: 186
• Page End: e68
• Publication Date: 2018-03-25
• Subjects: Veterinary dermatology -- Periodicals; Pet medicine -- Periodicals; 636.08965
• DOI: 10.1111/vde.12534
• Languages: English
• ISSNs: 0959-4493

Abstract

Abstract : Background: Pseudomonas aeruginosa is a challenging pathogen cultured from cases of acute and chronic canine otitis and sometimes in cases of deep pyoderma. The spread of antimicrobial resistance, especially carbapenem resistance, is a serious therapeutic challenge worldwide. Hypothesis/Objectives: To investigate the identification and characterization of resistant P. aeruginosa clinical canine isolates. Materials: Clinical isolates (n = 80) were collected from dogs with pyoderma (n = 18) and otitis (n = 62) in Korea. Methods: Antimicrobial susceptibility was determined using agar dilution and using Clinical and Laboratory Standards Institute guidelines for recording susceptibility for human Pseudomonas isolates; genetic relatedness of isolates was investigated by multilocus sequence typing (MLST) and Spe I macrorestriction analysis. The class 1 integrons were amplified and sequenced using primer walking. Results: Most isolates were susceptible to colistin (97.5%), polymyxin B (96.3%), ciprofloxacin (81.3%) and meropenem (80.0%); whereas resistance to aztreonam (80%), piperacillin (52.5%), piperacillin/tazobactam (41.3%) and cefepime (37.5%) was high; 12 carbapenem‐nonsusceptible isolates (15%) were detected. MLST revealed 45 different sequence types (STs) and macrorestriction analysis detected 55 distinct pulsotypes (PTs), which were divided into 25 clonal groups. Among carbapenem‐nonsusceptible isolates, 10 (83.3%) were VIM‐2‐producing strains. Nine VIM‐2‐producing isolates were identified as ST1047 and harboured the same 2.8 kb class 1 integron. One remaining isolate was ST1203 with 2.1 kb class 1 integron. Conclusions and clinical importance: This study demonstrated the diversity of the phenotype and genotype of clinical P. aeruginosa isolates from dogs with pyoderma and otitis. The identification of VIM‐2‐producing P. aeruginosa in dogs is alarming and warrants further surveillance. Abstract : Background – Pseudomonas aeruginosa is a challenging pathogen cultured from cases of acute and chronic canine otitis and sometimes in cases of deep pyoderma. The spread of antimicrobial resistance, especially carbapenem resistance, is a serious therapeutic challenge worldwide.Hypothesis/Objectives – To investigate the identification and characterization of resistant P. aeruginosa clinical canine isolates.Conclusions and clinical importance – This study demonstrated the diversity of the phenotype and genotype of clinical P. aeruginosa isolates from dogs with pyoderma and otitis. The identification of VIM‐2‐producing P. aeruginosa in dogs is alarming and warrants further surveillance.