Whole‐Cell Cascade Biotransformations for One‐Pot Multistep Organic Synthesis. Issue 10 (23rd February 2018)

• Record Type: Journal Article
• Authors: Wu, Shuke; Li, Zhi
• Is Part Of: ChemCatChem. Volume 10:Issue 10(2018)
• Page Start: 2164
• Page End: 2178
• Publication Date: 2018-02-23
• Subjects: biocatalysis -- cascade reactions -- enantioselectivity -- enzyme catalysis -- whole-cell biotransformation; Catalysis -- Periodicals; 541.39505
• DOI: 10.1002/cctc.201701669
• Languages: English
• ISSNs: 1867-3880

Abstract

Abstract: Performing one‐pot multi‐catalysis reactions is a revolutionary tool for multistep synthesis, representing a fast‐developing theme in catalysis field. To develop cascade reactions, enzymes are ideal catalysts due to their compatible reaction conditions. The implementation of enzyme cascades in recombinant microbial cells provides easily available self‐replicating catalysts for facile one‐pot biotransformations to produce a wide range of high‐value (chiral) chemicals. In this minireview, we summarize the recent development of whole‐cell cascade biotransformations according to the types of substrates, ranging from petrochemicals to biochemicals. Furthermore, we provide general instructions for the establishment of in vivo enzyme cascades, discuss the encountered problems and the corresponding solutions, and highlight the promising directions for future advance. Abstract : Bugs for Cascade Catalysis : Recombinant Escherichia coli is easily available and self‐replicating whole‐cell catalyst for biotransformations. Multiple enzymes could be co‐expressed in a single E. coli host, thus enabling facile one‐pot synthesis of valuable (chiral) products. In this paper, we reviewed the recent development, provided instruction, and discussed problems and solutions of whole‐cell cascade biotransformations.