Mactosylceramide prevents glial cell overgrowth by inhibiting insulin and fibroblast growth factor receptor signaling. Issue 11 (31st March 2017)

• Record Type: Journal Article
• Authors: Gerdøe‐Kristensen, Stine; Lund, Viktor K.; Wandall, Hans H.; Kjaerulff, Ole
• Is Part Of: Journal of cellular physiology. Volume 232:Issue 11(2017:Nov.)
• Page Start: 3112
• Page End: 3127
• Publication Date: 2017-03-31
• Subjects: cell growth -- glia -- glioblastoma -- sphingolipids; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.25762
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : Receptor tyrosine kinase (RTK) signaling controls key aspects of cellular differentiation, proliferation, survival, metabolism, and migration. Deregulated RTK signaling also underlies many cancers. Glycosphingolipids (GSL) are essential elements of the plasma membrane. By affecting clustering and activity of membrane receptors, GSL modulate signal transduction, including that mediated by the RTK. GSL are abundant in the nervous system, and glial development in Drosophila is emerging as a useful model for studying how GSL modulate RTK signaling. Drosophila has a simple GSL biosynthetic pathway, in which the mannosyltransferase Egghead controls conversion of glucosylceramide (GlcCer) to mactosylceramide (MacCer). Lack of elongated GSL in egghead ( egh ) mutants causes overgrowth of subperineurial glia (SPG), largely due to aberrant activation of phosphatidylinositol 3‐kinase (PI3K). However, to what extent this effect involves changes in upstream signaling events is unresolved. We show here that glial overgrowth in egh is strongly linked to increased activation of Insulin and fibroblast growth factor receptors (FGFR). Glial hypertrophy is phenocopied when overexpressing gain‐of‐function mutants of the Drosophila insulin receptor (InR) and the FGFR homolog Heartless (Htl) in wild type SPG, and is suppressed by inhibiting Htl and InR activity in egh . Knockdown of GlcCer synthase in the SPG fails to suppress glial overgrowth in egh nerves, and slightly promotes overgrowth in wild type, suggesting that RTK hyperactivation is caused by absence of MacCer and not by GlcCer accumulation. We conclude that an early product in GSL biosynthesis, MacCer, prevents inappropriate activation of insulin and fibroblast growth factor receptors in Drosophila glia. Abstract : Receptor tyrosine kinase signaling controls key aspects of cellular differentiation, proliferation, survival, metabolism, and migration. Glycosphingolipids modulate signal transduction, including that mediated by the receptor tyrosine kinases. We report that an early product in glycosphingolipid biosynthesis, mactosylceramide, prevents inappropriate activation of insulin and fibroblast growth factor receptors in Drosophila glial cells.