High spatiotemporal resolution bSSFP imaging of hyperpolarized [1‐13C]pyruvate and [1‐13C]lactate with spectral suppression of alanine and pyruvate‐hydrate. Issue 3 (16th February 2018)

• Record Type: Journal Article
• Authors: Milshteyn, Eugene; von Morze, Cornelius; Gordon, Jeremy W.; Zhu, Zihan; Larson, Peder E.Z.; Vigneron, Daniel B.
• Is Part Of: Magnetic resonance in medicine. Volume 80:Issue 3(2018)
• Page Start: 1048
• Page End: 1060
• Publication Date: 2018-02-16
• Subjects: 13C -- hyperpolarized -- metabolism -- pyruvate -- SSFP; Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.27104
• Languages: English
• ISSNs: 0740-3194

Abstract

Abstract : Purpose: The balanced steady‐state free precession (bSSFP) acquisition enables high spatiotemporal resolution for hyperpolarized 13 C MRI at 3T but is limited by spectral contamination from adjacent resonances. The purpose of this study was to develop a framework for in vivo dynamic high resolution imaging of hyperpolarized [1‐ 13 C]pyruvate and [1‐ 13 C]lactate generated in vivo at 3T by simplifying the spectrum through the use of selective suppression pulses. Methods: Spectral suppression pulses were incorporated into the bSSFP sequence for suppression of [1‐ 13 C]alanine and [1‐ 13 C]pyruvate‐hydrate signals, leaving only the pyruvate and lactate resonances. Subsequently, the bSSFP pulse width, time‐bandwidth, and repetition time were optimized for imaging these dual resonances. Results: The spectral suppression reduced both the alanine and pyruvate‐hydrate signals by 85.5 ± 4.9% and had no significant effect on quantitation of pyruvate to lactate conversion (liver: P = 0.400, kidney: P = 0.499). High resolution (2 × 2 mm 2 and 3 × 3 mm 2 ) subsecond 2D coronal projections and 3D 2.5 mm isotropic images were obtained in rats and tumor‐bearing mice with 1.8–5 s temporal resolution, allowing for calculation of lactate‐to‐pyruvate ratios and k PL . Conclusion: The developed framework presented here shows the capability for dynamic high resolution volumetric hyperpolarized bSSFP imaging of pyruvate‐to‐lactate conversion on a clinical 3T MR scanner.