Carbonic anhydrase II microcrystals suitable for XFEL studies. Issue 6 (5th June 2018)

• Record Type: Journal Article
• Authors: Lomelino, Carrie L.; Kim, Jin Kyun; Lee, Cheol; Lim, Seon Woo; Andring, Jacob T.; Mahon, Brian P.; Chung, Moses; Kim, Chae Un; McKenna, Robert
• Is Part Of: Acta crystallographica. Volume 74:Issue 6(2018:Jun.)
• Page Start: 327
• Page End: 330
• Publication Date: 2018-06-05
• Subjects: X‐ray free‐electron lasers -- XFELs -- serial femtosecond crystallography -- time‐resolved crystallography -- microcrystals -- carbonic anhydrase II; Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X18006118
• Languages: English
• ISSNs: 2053-230X

Abstract

Abstract : Carbonic anhydrase II microcrystals suitable for XFEL data collection diffracted to 1.7 Å resolution and were indexed in space group P 21, with unit‐cell parameters a = 42.2, b = 41.2, c = 72.0 Å, β = 104.2°. Abstract : Recent advances in X‐ray free‐electron laser (XFEL) sources have permitted the study of protein dynamics. Femtosecond X‐ray pulses have allowed the visualization of intermediate states in enzyme catalysis. In this study, the growth of carbonic anhydrase II microcrystals (40–80 µm in length) suitable for the collection of XFEL diffraction data at the Pohang Accelerator Laboratory is demonstrated. The crystals diffracted to 1.7 Å resolution and were indexed in space group P 21, with unit‐cell parameters a = 42.2, b = 41.2, c = 72.0 Å, β = 104.2°. These preliminary results provide the necessary framework for time‐resolved experiments to study carbonic anhydrase catalysis at XFEL beamlines.