Petroleum Degradation, Biosurfactant and Laccase Production by Fusarium neocosmosporiellum RH-10: A Microcosm Study. Issue 4 (19th May 2018)

• Record Type: Journal Article
• Authors: Azin, Ehsan; Moghimi, Hamid; Heidarytabar, Rezvan
• Is Part Of: Soil & sediment contamination. Volume 27:Issue 4(2018)
• Page Start: 329
• Page End: 342
• Publication Date: 2018-05-19
• Subjects: Biosurfactant -- crude oil -- microcosm study -- mycoremediation -- Fusariumneocosmosporiellum -- laccase enzyme; Soil pollution -- Periodicals; Soil protection -- Periodicals; 628.5505
• DOI: 10.1080/15320383.2018.1473334
• Languages: English
• ISSNs: 1532-0383

Abstract

ABSTRACT: The aim of this study was to evaluate the potential of crude oil removal by fungal strains isolated from Arak refinery. The results showed that the RH10 strain is a potent strain as a surfactant producer and degrader of petrochemical hydrocarbons. The strain was identified as a Fusarium neocosmosporiellum and could degrade 58% of hydrocarbons in the minimal medium and reduce the surface tension from 45 to 26.5 mN m −1 . Moreover, residual crude oil analysis with Fourier transform infrared spectrophotometry showed that this strain was able to degrade 50% of aliphatic compounds. To investigate the mechanism of degradation, oxidase enzymes were assayed and it was found that F. neocosmosporiellum can produce 1.94 U L −1 of laccase in 10 g L −1 crude oil. Carbon, nitrogen, phosphorus and soil pattern optimization in a microcosm study showed that this strain removed 44% and 27% of the crude oil from contaminated soil in 1% and 5% crude oil concentrations, respectively. Under optimum condition, 9.66 g kg −1 crude oil was removed by F. neocosmosporiellum when the initial oil concentration was 50 g kg −1, at the end of 150 days microcosm experiment. The results demonstrated the promising potential of fungi strain for cleaning of contaminated soil.