Comparative analysis of Klebsiella pneumoniae strains isolated in 2012–2016 that differ by antibiotic resistance genes and virulence genes profiles. (3rd April 2018)

• Record Type: Journal Article
• Authors: Lev, Anastasia I.; Astashkin, Eugeny I.; Kislichkina, Angelina A.; Solovieva, Ekaterina V.; Kombarova, Tatiana I.; Korobova, Olga V.; Ershova, Olga N.; Alexandrova, Irina A.; Malikov, Vladimir E.; Bogun, Alexander G.; Borzilov, Alexander I.; Volozhantsev, Nikolay V.; Svetoch, Edward A.; Fursova, Nadezhda K.
• Is Part Of: Pathogens and global health. Volume 112:Number 3(2018)
• Page Start: 142
• Page End: 151
• Publication Date: 2018-04-03
• Subjects: Klebsiella pneumoniae -- antibacterial resistance -- virulence -- MLST -- K-serotype; Communicable diseases -- Periodicals; Public health -- International cooperation -- Periodicals; World health -- Periodicals; 362.1969
• DOI: 10.1080/20477724.2018.1460949
• Languages: English
• ISSNs: 2047-7724

Abstract

Abstract: The antibacterial resistance and virulence genotypes and phenotypes of 148 non-duplicate Klebsiella pneumoniae strains collected from 112 patients in Moscow hospitals in 2012–2016 including isolates from the respiratory system (57%), urine (30%), wounds (5%), cerebrospinal fluid (4%), blood (3%), and rectal swab (1%) were determined. The majority (98%) were multidrug resistant (MDR) strains carrying bla SHV (91%), bla CTX-M (74%), bla TEM (51%), bla OXA (38%), and bla NDM (1%) beta-lactamase genes, class 1 integrons (38%), and the porin protein gene ompK36 (96%). The beta-lactamase genes bla TEM-1, bla SHV-1, bla SHV-11, bla SHV-110, bla SHV-190, bla CTX-M-15, bla CTX-M-3, bla CTX-M-55, bla OXA-48, bla OXA-244, and bla NDM-1 were detected; class 1 integron gene cassette arrays ( aadA1 ), ( dfrA7 ), ( dfrA1-orfC ), ( aadB-aadA1 ), ( dfrA17-aadA5 ), and ( dfrA12-orfF-aadA2 ) were identified. Twenty-two (15%) of clinical K. pneumoniae strains had hypermucoviscous (HV) phenotype defined as string test positive. The rmpA gene associated with HV phenotype was detected in 24% of strains. The intrapersonal mutation of rmpA gene (deletion of one nucleotide at the polyG tract) was a reason for negative hypermucoviscosity phenotype and low virulence of rmpA -positive K. pneumoniae strain KPB584. Eighteen virulent for mice strains with LD50 ≤ 10 4 CFU were attributed to sequence types ST23, ST86, ST218, ST65, ST2174, and ST2280 and to capsular types K1, K2, and K57. This study is the first report about hypervirulent K. pneumoniae strain KPB2580-14 of ST23 K1 harboring extended-spectrum beta-lactamase CTX-M-15 and carbapenemase OXA-48 genes located on pCTX-M-15-like and pOXA-48-like plasmids correspondingly.