Assessment of induced CYP3A activity in pregnant women using 4β-hydroxycholesterol: Cholesterol ratio as an appropriate metabolic marker. Issue 3 (June 2018)

• Record Type: Journal Article
• Authors: Kim, Andrew HyoungJin; Kim, Bora; Rhee, Su-jin; Lee, Yujin; Park, Joong Shin; Lee, Seung Mi; Kim, Sun Min; Lee, SeungHwan; Yu, Kyung-Sang; Jang, In-Jin; Cho, Joo-Youn
• Is Part Of: Drug metabolism and pharmacokinetics. Volume 33:Issue 3(2018)
• Page Start: 173
• Page End: 178
• Publication Date: 2018-06
• Subjects: Pregnancy -- Cytochrome P450 3A -- Metabolic markers -- 4β-hydroxycholesterol/cholesterol -- GC-MS; Drugs -- Metabolism -- Periodicals; Pharmacokinetics -- Periodicals; 615.7
• DOI: 10.1016/j.dmpk.2018.04.004
• Languages: English
• ISSNs: 1347-4367

Abstract

Abstract: Aims: This study was aimed at evaluating changes in CYP3A activity following and during pregnancy by analyzing metabolic markers for CYP3A activity, which can help avoid unnecessary drug exposure and invasive sampling. Methods: Forty-eight pregnant women and 25 non-pregnant women were enrolled in this study. Plasma and urine samples were collected from the pregnant women during each trimester and from the non-pregnant women for evaluation of metabolic markers for CYP3A activity. Metabolic markers for CYP3A activity were measured using GC-MS. Results: An increased 4β-hydroxycholesterol/cholesterol ratio, consistent with high CYP3A activity, was observed in pregnant women compared with that in non-pregnant women; however, no differences were observed among trimesters. No significant differences were observed in urinary markers. Conclusions: We observed an increase in the activity of CYP3A following but not during pregnancy when measured using the 4β-hydroxycholesterol/cholesterol ratio. In addition, based on our results, we suggest that the plasma 4β-hydroxycholesterol/cholesterol ratio be used to measure CYP3A activity in pregnant women. Graphical abstract: