LncRNA n335586/miR-924/CKMT1A axis contributes to cell migration and invasion in hepatocellular carcinoma cells. (10th August 2018)

• Record Type: Journal Article
• Authors: Fan, Hongxia; Lv, Ping; Mu, Ting; Zhao, Xiaopei; Liu, Yankun; Feng, Yujie; Lv, Jing; Liu, Min; Tang, Hua
• Is Part Of: Cancer letters. Volume 429(2018)
• Page Start: 89
• Page End: 99
• Publication Date: 2018-08-10
• Subjects: lncRNA n335586 -- CKMT1A -- HCC migration/invasion -- miR-924 -- ceRNA; ceRNA competing endogenous RNA -- CKMT1A creatine kinase mitochondrial 1A -- EMT epithelial-mesenchymal transition -- HBV hepatitis B virus -- HBx hepatitis B virus X protein -- HBx-LINE1 HBx-long interspersed nuclear element1 -- HCC hepatocellular carcinoma -- LET low expression in tumor -- lncRNAs long noncoding RNAs -- lncRNA-Dreh lncRNA down-regulated expression by HBx -- HOTTIP HOXA transcript at the distal tip -- HULC highly up-regulated in liver cancer -- MALAT-1 metastasis-associated lung adenocarcinoma transcript 1 -- MEG3 maternally expressed gene-3 -- miRNA microRNA -- MREs miRNAs response elements -- MVIH lncRNA associated with microvascular invasion in HCC -- UCA1 urothelial carcinoma associated 1; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2018.05.010
• Languages: English
• ISSNs: 0304-3835

Abstract

Abstract: Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide and chronic hepatitis B virus (HBV) infection is a major risk factor for HCC. Emerging evidences indicate that long noncoding RNAs (lncRNAs) play a pivotal role in HCC development, but its contribution to HBV-related HCC remains largely unclear. Differentially expressed lncRNAs in HBV-related HCC tissues were identified by deep sequencing in our previous study. The function of lncRNA n335586, one of most up-regulated lncRNAs in HBV-related HCC, was characterized in the present study. We found that the expression of n335586 was significantly increased in HBV positive HCC tissues and cells and was induced by HBV in vitro . Function study indicated that lncRNA n335586 remarkably promoted HCC cells migration, invasion and epithelial-mesenchymal transition (EMT) in vitro and metastasis in vivo . Further mechanistic studies showed lncRNA n335586 promoted HCC cells migration and invasion through facilitating the expression of its host gene CKMT1A by competitively binding miR-924. In conclusion, we demonstrated that the n335586/miR-924/CKMT1A axis contributes to HCC cell migration and invasion, which may be helpful for understanding of pathogenesis of HBV-related HCC. Highlights: The expression of lncRNA n335586 is induced by HBV. lncRNA n335586 promotes HCC cells migration/invasion in vitro and metastasis in vivo . lncRNA n335586 increases its host gene CKMT1A level by competitively binding miR-924. lncRNA n335586/miR-924/CKMT1A axis contributes to HCC cell migration and invasion.