Improvement and modeling of culture parameters to enhance biomass and lipid production by the oleaginous yeast Cryptococcus curvatus grown on acetate. (September 2015)

• Record Type: Journal Article
• Authors: Béligon, Vanessa; Poughon, Laurent; Christophe, Gwendoline; Lebert, André; Larroche, Christian; Fontanille, Pierre
• Is Part Of: Bioresource technology. Volume 192(2015)
• Page Start: 582
• Page End: 591
• Publication Date: 2015-09
• Subjects: Volatile fatty acids -- Cryptococcus curvatus -- Microbial lipids -- Bioconversion -- Biodiesel; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.06.041
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: pH 7 enhances the growth rate of Cryptococcus curvatus cultivated on acetate. New pH regulation system dispensing acetate allows high-cell density culture. Lipid concentration is multiplied by 5.68 when the medium exhibits a C/N ratio of 300. Higher C/N ratios do not induce drastic increase of lipid content. Volatile fatty acids can be converted into lipids, usable as biodiesel feedstock. Abstract: The improvement of culture parameters for lipid production from acetate as carbon source was investigated using the oleaginous yeast Cryptococcus curvatus . A new pH regulation system dispensing acetate was developed for fed-batch culture and allowed obtaining nearly 80 g/L biomass within 60 h with a maximal growth rate of 0.28 h −1 . A biological model was developed from experimental data. The influence of three C/N ratios of 300, 500 and 900 were tested during a multi-phases process on lipid accumulation. The C/N ratio of 300 was reported to be the most suitable for lipid storage. No significant increase of lipids content was obtained with higher value. A maximal content of 60% DCW of lipid was obtained. The determination of fatty acids profiles of the microbial oils has confirmed that the valorization of acetate by microbial oils production was a promising perspective.