9.4T and 17.6T MRI of Retinoblastoma: Ex Vivo evaluation of microstructural anatomy and disease extent compared with histopathology. Issue 6 (28th November 2017)

• Record Type: Journal Article
• Authors: de Jong, Marcus C.; de Graaf, Pim; Pouwels, Petra J.W.; Beenakker, Jan‐Willem; Jansen, Robin W.; Geurts, Jeroen J.G.; Moll, Annette C.; Castelijns, Jonas A.; van der Valk, Paul; van der Weerd, Louise
• Is Part Of: Journal of magnetic resonance imaging. Volume 47:Issue 6(2018)
• Page Start: 1487
• Page End: 1497
• Publication Date: 2017-11-28
• Subjects: retinoblastoma -- magnetic resonance imaging -- 9.4 Tesla -- 17.6 Tesla -- optic nerve -- tumor seeding -- diagnostic accuracy -- morphology -- MR microscopy; Magnetic resonance imaging -- Periodicals; 616
• DOI: 10.1002/jmri.25913
• Languages: English
• ISSNs: 1053-1807

Abstract

Abstract : Background: Retinoblastoma is the most common intraocular tumor in childhood with a good prognosis in terms of mortality, but detailed information about tumor morphology and disease extent in retinoblastoma is important for treatment decision making. Purpose: To demonstrate ultrahigh‐field MRI tumor morphology and tumor extent in retinoblastoma correlating with in and ex vivo images with histopathology. Study Type: Prospective case series. Population: Six retinoblastoma patients (median age 5.5 months, range 2–14) were prospectively included in this study. Median time between diagnosis and enucleation was 8 days (range 7–19). Field Strength/Sequence: In vivo pre‐enucleation at 1.5T MRI with a circular surface coil. Ex vivo imaging (FLASH T1 ‐weighted and RARE T2 ‐weighted) was performed at field strengths of 9.4T and 17.6T. Assessment: After ex vivo imaging, the eyes were histopathologically analyzed and morphologically matched with MRI findings by three authors (two with respectively 14 and 4 years of experience in ocular MRI and one with 16 years of experience in ophthalmopathology). Results: Small submillimeter morphological aspects of intraocular retinoblastoma were successfully depicted with higher‐resolution MRI and matched with histopathology images. With ex vivo MRI a small subretinal tumor seed (300 μm) adjacent to the choroid was morphologically matched with histopathology. Also, a characteristic geographical pattern of vital tumor tissue (400 μm) surrounding a central vessel interspersed with necrotic areas correlated with histopathology images. Tumor invasion into the optic nerve showed a higher signal intensity on T1 ‐weighted higher‐resolution MRI. Data Conclusion: Higher‐resolution MRI allows for small morphological aspects of intraocular retinoblastoma and extraocular disease extent not visible on currently used clinical in vivo MRI to be depicted. Level of Evidence: 4 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2018;47:1487–1497.