Ultra-High-Throughput Sample Preparation System for Lymphocyte Immunophenotyping Point-of-Care Diagnostics. (October 2016)

• Record Type: Journal Article
• Authors: Walsh, David I.; Murthy, Shashi K.; Russom, Aman
• Is Part Of: Journal of laboratory automation. Volume 21:Number 5(2016:Oct.)
• Page Start: 706
• Page End: 712
• Publication Date: 2016-10
• Subjects: centrifugal microfluidics -- paper-based microfluidics -- cell capture -- sample preparation -- cell immunophenotyping; Medical laboratory technology -- Periodicals; Laboratories -- Equipment and supplies -- Periodicals; Diagnosis, Laboratory -- Periodicals; 616.075
• DOI: 10.1177/2211068216634003
• Languages: English
• ISSNs: 2211-0682

Abstract

Point-of-care (POC) microfluidic devices often lack the integration of common sample preparation steps, such as preconcentration, which can limit their utility in the field. In this technology brief, we describe a system that combines the necessary sample preparation methods to perform sample-to-result analysis of large-volume (20 mL) biopsy model samples with staining of captured cells. Our platform combines centrifugal-paper microfluidic filtration and an analysis system to process large, dilute biological samples. Utilizing commercialization-friendly manufacturing methods and materials, yielding a sample throughput of 20 mL/min, and allowing for on-chip staining and imaging bring together a practical, yet powerful approach to microfluidic diagnostics of large, dilute samples.