Biomek Cell Workstation: A Variable System for Automated Cell Cultivation. (June 2016)

• Record Type: Journal Article
• Authors: Lehmann, R.; Severitt, J. C.; Roddelkopf, T.; Junginger, S.; Thurow, K.
• Is Part Of: Journal of laboratory automation. Volume 21:Number 3(2016:Jun.)
• Page Start: 439
• Page End: 450
• Publication Date: 2016-06
• Subjects: life science automation -- automated cell culturing -- suspension cells -- Biomek Cell Workstation; Medical laboratory technology -- Periodicals; Laboratories -- Equipment and supplies -- Periodicals; Diagnosis, Laboratory -- Periodicals; 616.075
• DOI: 10.1177/2211068215599786
• Languages: English
• ISSNs: 2211-0682

Abstract

Automated cell cultivation is an important tool for simplifying routine laboratory work. Automated methods are independent of skill levels and daily constitution of laboratory staff in combination with a constant quality and performance of the methods. The Biomek Cell Workstation was configured as a flexible and compatible system. The modified Biomek Cell Workstation enables the cultivation of adherent and suspension cells. Until now, no commercially available systems enabled the automated handling of both types of cells in one system. In particular, the automated cultivation of suspension cells in this form has not been published. The cell counts and viabilities were nonsignificantly decreased for cells cultivated in AutoFlasks in automated handling. The proliferation of manual and automated bioscreening by the WST-1 assay showed a nonsignificant lower proliferation of automatically disseminated cells associated with a mostly lower standard error. The disseminated suspension cell lines showed different pronounced proliferations in descending order, starting with Jurkat cells followed by SEM, Molt4, and RS4 cells having the lowest proliferation. In this respect, we successfully disseminated and screened suspension cells in an automated way. The automated cultivation and dissemination of a variety of suspension cells can replace the manual method.