Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System. (June 2016)

• Record Type: Journal Article
• Authors: Kong, Ling X.; Perebikovsky, Alexandra; Moebius, Jacob; Kulinsky, Lawrence; Madou, Marc
• Is Part Of: Journal of laboratory automation. Volume 21:Number 3(2016:Jun.)
• Page Start: 323
• Page End: 355
• Publication Date: 2016-06
• Subjects: analytical -- assay -- point-of-care -- centrifugal -- microfluidics -- sample-to-answer -- LoD; Medical laboratory technology -- Periodicals; Laboratories -- Equipment and supplies -- Periodicals; Diagnosis, Laboratory -- Periodicals; 616.075
• DOI: 10.1177/2211068215588456
• Languages: English
• ISSNs: 2211-0682

Abstract

The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.