High-fat diet exposure, regardless of induction of obesity, is associated with altered expression of genes critical to normal ovulatory function. (15th July 2018)

• Record Type: Journal Article
• Authors: Hohos, Natalie M.; Cho, Kirstin J.; Swindle, Delaney C.; Skaznik-Wikiel, Malgorzata E.
• Is Part Of: Molecular and cellular endocrinology. Volume 470(2018)
• Page Start: 199
• Page End: 207
• Publication Date: 2018-07-15
• Subjects: Ovary -- High-fat diet -- Obesity -- Female fertility -- RNA sequencing; Transcription factor 3 Atf3 -- A disintegrin-like and metallopeptidase with thrombospondin type 1 motif, 1 Adamts1 -- Activating Anti-mullerian hormone AMH -- B cell translocation gene 2 Btg2 -- Cysteine rich protein 61 Cry61 -- Differentially expressed DE -- Diet-induced obesity DIO -- Endothelin 2 Edn2 -- ERBB receptor feedback inhibitor 1 Errfi1 -- Follicle-stimulating hormone FSH -- FBJ osteosarcoma oncogene B Fosb -- High-fat/High-sugar diet HF/HSD -- High-fat diet HFD -- High-fat diet lean HF-Ln -- High-fat diet obese HF-Ob -- Leptin Lep -- Luteinizing hormone LH -- National Hormone and Peptide Program NHPP -- Nuclear factor, interleukin 3, regulated Nfil3 -- Nuclear receptor subfamily 4, group A, member 1 Nr4a1 -- Protein kinase, cGMP-dependent, type II Prkg2 -- Standard error of the mean SEM -- Suppressor of cytokine signaling 3 Socs3 -- Thyroid stimulating hormone TSH -- Tumor necrosis factor alpha induced protein 6 Tnfaip6 -- Quantitative reverse transcription polymerase chain reaction qRT-PCR; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2017.10.016
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: We evaluated the impact of high-fat diet (HFD) on ovarian gene expression. Female 5-week-old C57BL/6J mice were fed a 60% HFD or standard chow for 10 weeks. HFD-fed mice were then separated into obese (HF-Ob) and lean (HF-Ln) based on body weight. HFD exposure led to impairment of the estrous cycle, changes in hormones affecting reproduction, and decreased primordial follicles regardless of the development of obesity. RNA-sequencing of whole ovaries identified multiple genes with altered expression after HFD, with 25 genes displaying decreased expression in both HF-Ln and HF-Ob mice compared to the chow-fed controls (q < 0.05). Several of these 25 genes are involved in normal ovarian functions, including ovulation ( Edn2, Tnfaip6, Errfi1, Prkg2, and Nfil3 ), luteinization ( Edn2 ), and luteolysis ( Nr4a1 ). Taken together, elevated dietary fat intake, regardless of obesity, is associated with impaired estrous cycle, depletion of the ovarian reserve, and altered expression of genes critical to normal ovulatory function. Highlights: HFD alters the expression of ovarian genes involved in normal ovulatory function, irrespective of obesity. HFD negatively impacts ovarian reserve. HFD is associated with estrous cycle disruption - finding suggestive of an abnormal ovulatory function.