Response of serum 25(OH)D to Vitamin D and calcium supplementation in school-children from a semi-rural setting in India. Issue 180 (June 2018)

• Record Type: Journal Article
• Authors: Mandlik, Rubina; Khadilkar, Anuradha; Kajale, Neha; Ekbote, Veena; Patwardhan, Vivek; Mistry, Sejal; Khadilkar, Vaman; Chiplonkar, Shashi
• Is Part Of: Journal of steroid biochemistry and molecular biology. Issue 180(2017)
• Page Start: 35
• Page End: 40
• Publication Date: 2018-06
• Subjects: Vitamin D -- Deficiency -- Sufficiency -- Daily supplementation -- Optimal concentrations; Steroid hormones -- Periodicals; Biochemistry -- Periodicals; Hormones -- Periodicals; Molecular Biology -- Periodicals; Hormones stéroïdes -- Périodiques; Steroid hormones; Periodicals; 572.579
• DOI: 10.1016/j.jsbmb.2017.12.003
• Languages: English
• ISSNs: 0960-0760

Abstract

Highlights: Vitamin D-Calcium supplementation (1000IU–500 mg/day) effective in achieving sufficiency. Children with baseline 25(OH)D concentrations <45 nmol/L experience most benefit. Basal 25(OH)D concentrations inversely impact improvement post-supplementation. Abstract: The objectives of this study were to: 1) Determine the impact of varying baseline serum 25OHD on increase in vitamin D concentrations after daily supplementation with vitamin D and calcium (1000 IU + 500 mg respectively) for six months in school-children from a semi-rural setting 2) Test the efficacy of daily vitamin D-calcium supplementation on improvement in serum vitamin D concentrations to ≥75 nmol/L. Data collected from 106 subjects (58 boys, 48 girls), aged 6–12 years, included anthropometric measures like height and weight, body composition analysis, three one-day dietary recalls and sunlight exposure (by questionnaire). Blood was collected at baseline and endline and estimated for serum vitamin D by ELISA technique using standard kits. Classification of Vitamin D status was performed according to the 2011 Endocrine Society Practice Guidelines: vitamin D deficiency – <50 nmol/L; insufficiency – 50.0–74.9 nmol/L; sufficiency – ≥75 nmol/L. Statistical analysis was performed using SPSS software. Mean baseline serum vitamin D concentration was 59.7 ± 11.2 nmol/L; this rose to 79.8 ± 23.3 nmol/L with no significant differences between genders at the two time-points. Inverse relationship was obtained between baseline serum 25(OH)D concentrations and change in serum concentrations after supplementation, implying that with increasing baseline serum concentrations of 25(OH)D, increase in vitamin D levels post supplementation were significantly lower (r = − 0.96, p < 0.0001). Greatest benefit of change in serum vitamin D concentrations after supplementation was experienced by children with basal concentrations of <45 nmol/L. Daily vitamin D supplementation was effective in improving serum 25(OH)D to ≥75 nmol/L in 44% of children. Significantly higher percentage of children who were deficient at baseline (64%) were able to attain serum concentrations of ≥75 nmol/L as compared to children who were vitamin D insufficient (43%) ( p < 0.001). Thus, daily supplementation with 1000 IU of vitamin D along with 500 mg of calcium helped in improving serum vitamin D concentrations to ≥75 nmol/L. Children who were vitamin D deficient particularly experienced these benefits.