Chitosan-functionalised single-walled carbon nanotube-mediated drug delivery of SNX-2112 in cancer cells. (September 2016)

• Record Type: Journal Article
• Authors: Zheng, Lixia; Wu, Shao; Tan, Li; Tan, Huo; Yu, Baodan
• Is Part Of: Journal of biomaterials applications. Volume 31:Number 3(2016:Sep.)
• Page Start: 379
• Page End: 386
• Publication Date: 2016-09
• Subjects: Single-walled carbon nanotubes -- chitosan -- drug delivery -- SNX-2112 -- lactate dehydrogenase -- reactive oxygen species -- cancer; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1177/0885328216651183
• Languages: English
• ISSNs: 0885-3282

Abstract

Delivery of amphiphobic drugs (insoluble in both water and oil) has been a great challenge in drug delivery. SNX-2112, a novel inhibitor of Hsp90, is a promising drug candidate for treating various types of cancers; however, the insolubility greatly limits its clinical application. This study aimed to build a new type of drug delivery system using single-walled carbon nanotubes (SWNTs) for controllable release of SNX-2112; chitosan (CHI) was non-covalently added to SWNTs to improve their biocompatibility. SWNTs-CHI demonstrated high drug-loading capability; the release of SNX-2112 was pH triggered and time related. The intracellular reactive oxygen species of SWNTs–CHI increased, compared with that of SWNTs, leading to higher mitogen-activated protein kinase and cell apoptosis. The results of western-blotting, lactate dehydrogenase (LDH) release assay, and cell viability assay analyses indicated that apoptosis-related proteins were abundantly expressed in K562 cells and that the drug delivery system significantly inhibited K562 cells. Thus, SWNT–CHI/SNX-2112 shows great potential as a drug delivery system for cancer therapy.