Development of an in situ‐Product Crystallization (ISPC)‐Concept to Shift the Reaction Equilibria of Selected Amine Transaminase‐Catalyzed Reactions. Issue 18 (11th May 2018)

• Record Type: Journal Article
• Authors: Hülsewede, Dennis; Tänzler, Marco; Süss, Philipp; Mildner, Andrea; Menyes, Ulf; von Langermann, Jan
• Is Part Of: European journal of inorganic chemistry. Issue 18(2018)
• Page Start: 2130
• Page End: 2133
• Publication Date: 2018-05-11
• Subjects: Biocatalysis -- Amine synthesis -- Product removal -- Crystallization -- Chirality; Chemistry, Inorganic -- Periodicals; Organometallic chemistry -- Periodicals; Bioinorganic chemistry -- Periodicals; Solid state chemistry -- Periodicals; 546
• DOI: 10.1002/ejoc.201800323
• Languages: English
• ISSNs: 1434-1948

Abstract

Abstract : The synthesis of enantiopure amines via amine transaminases involves several challenges including unfavorable reaction equilibria and product inhibition. Described here is a non‐catalytic approach to overcome such problems by using an in situ‐product crystallization (ISPC) to selectively remove a targeted product amine from an amine transaminase‐catalyzed reaction. The continuous removal of the product amine from its reaction solution as a barely soluble salt effectively yields a displacement of the reaction equilibrium towards the products and facilitates a simple downstream processing approach via filtration. The targeted product amine is eventually obtained from the salt, while the counterion compound can be easily recycled. Abstract : A novel in situ‐product crystallization method has been developed to overcome the unfavorable equilibrium of transaminase‐catalyzed reactions. A specific carboxylic acid is added to the aqueous reaction mixture to form a barely soluble salt with the targeted product amine, which pushes the reaction to the product side and simplifies downstream‐processing.