Effects of Mad Honey on Some Biochemical Parameters in Rats. (October 2016)

• Record Type: Journal Article
• Authors: Sahin, Huseyin; Yildiz, Oktay; Kolayli, Sevgi
• Is Part Of: Journal of evidence-based complementary & alternative medicine. Volume 21:Number 4(2016:Oct.)
• Page Start: 255
• Page End: 259
• Publication Date: 2016-10
• Subjects: GTX-III -- mad honey -- blood biomarkers; Alternative medicine -- Periodicals; Complementary Therapies -- Periodicals; 615.505
• DOI: 10.1177/2156587215596430
• Languages: English
• ISSNs: 2156-5872

Abstract

The aims of this study were to determine grayanotoxin (GTX-III) toxin level in mad honey using liquid chromatography-tandem mass spectrometry and examine the dynamic changes of certain biochemical parameters in blood serum of rats that consumed mad honey. For the experimental animal study, 20 Sprague-Dawley female rats were divided into 5 groups of 4 rats each, with one group being the control group (Group 1) and the others being the experimental groups (Groups 2-5). Groups 2, 3, 4, and 5 were, respectively, given mad honey extract at doses of 0.3, 0.6, 1.2, and 2.4 mg/g body weight/day via oral gavage for 8 days. According to results, the quantity of GTX-III found in the honey sample as 39.949 ± 0.020 μg GTX-III/g honey, and the biochemical analysis of the tested parameters (aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, alkaline phosphatase, creatine kinase, and creatine kinase muscle and brain) showed a significant elevation with increasing concentration of honey. In conclusion, the use of increasing concentrations of Rhododendron honey was seen as a source of enzymatic symptoms.