Vitamin B12 and omega-3 fatty acids together regulate lipid metabolism in Wistar rats. (August 2015)

• Record Type: Journal Article
• Authors: Khaire, Amrita; Rathod, Richa; Kale, Anvita; Joshi, Sadhana
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 99(2015:Aug.)
• Page Start: 7
• Page End: 17
• Publication Date: 2015-08
• Subjects: ADP adiponectin -- ACC acetyl CoAcarboxylase -- CPT carnitine palmitoyltransferase -- DHA docosahexanoic acid -- EPA eicosapentaenoic acid; Acetyl CoA carboxylase-1 -- Adiponectin -- Carnitine palmitoyltransferase-1 -- Omega-3 fatty acids -- Triglycerides -- Vitamin B12; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2015.04.006
• Languages: English
• ISSNs: 0952-3278

Abstract

Abstract: Our recent study indicates that maternal vitamin B12 and omega-3 fatty acid status influence plasma and erythrocyte fatty acid profile in dams. The present study examines the effects of prenatal and postnatal vitamin B12 and omega-3 fatty acid status on lipid metabolism in the offspring. Pregnant dams were divided into five groups: Control; Vitamin B12 deficient (BD); Vitamin B12 supplemented (BS); Vitamin B12 deficient group supplemented with omega-3 fatty acids (BDO); Vitamin B12 supplemented group with omega-3 fatty acids (BSO). The offspring were continued on the same diets till 3 month of age. Vitamin B12 deficiency increased cholesterol levels ( p <0.01) but reduced docosahexaenoic acid (DHA) ( p <0.05), liver mRNA levels of acetyl CoA carboxylase-1 (ACC-1) ( p <0.05) and carnitine palmitoyltransferase-1 (CPT-1) ( p <0.01) in the offspring. Omega-3 fatty acid supplementation to this group normalized cholesterol but not mRNA levels of ACC-1 and CPT-1. Vitamin B12 supplementation normalized the levels cholesterol to that of control but increased plasma triglyceride ( p <0.01) and reduced liver mRNA levels of adiponectin, ACC-1, and CPT-1 ( p <0.01 for all). Supplementation of both vitamin B12 and omega-3 fatty acid normalized triglyceride and mRNA levels of all the above genes. Prenatal and postnatal vitamin B12 and omega-3 fatty acids together play a crucial role in regulating the genes involved in lipid metabolism in adult offspring.