Creating a Novel Graphene Oxide/Iron/Polylactic Acid Composite that Promotes Dental Pulp Stem Cell Proliferation and Mineralization. (16th April 2018)

• Record Type: Journal Article
• Authors: Isseroff, Rebecca; Chen, John; Khan, Zaiff; Guha, Anoushka; Lin, Simon; Li, Juyi; Fang, Kuan-che; Zhang, Linxi; Simon, Marcia; Rafailovich, Miriam
• Is Part Of: MRS advances. Volume 3:Number 30(2018)
• Page Start: 1725
• Page End: 1733
• Publication Date: 2018-04-16
• Subjects: bone, -- biomaterial, -- thin film; Electrical engineering -- Congresses; Physics -- Congresses; Materials -- Research -- Congresses; Materials science -- Congresses; 620.11
• DOI: 10.1557/adv.2018.364
• Languages: English
• ISSNs: 2059-8521

Abstract

ABSTRACT: Dental pulp stem cells (DPSCs) can differentiate into bone cells when provided the correct environment, potentially generating cells to repair non-union fractures. Polylactic Acid (PLA) is a biocompatible polymer for 3-D printing of scaffolds, but DPSCs do not proliferate well on PLA. With the goal of making PLA more conducive for DPSC growth, Graphene Oxide (GO); partially reduced Graphene Oxide (pRGO); GO with iron nanoparticles (FeGO) or Fe-pRGO were incorporated into PLA and spun cast as thin films onto silicon wafers for DPSC plating. DPSCs on Fe-pRGO displayed the fastest doubling time and the highest cell modulus; Fe-pRGO with exterior magnets produced high cell density. SEM demonstrated DPSC mineralization, whereas PLA-only DPSC cultures showed none. Results suggest that PLA/Fe-pRGO and PLA/pRGO enhance DPSC proliferation and possibly differentiation with the potential for use as a 3-D printed scaffold for tissue engineering.