Preparation and evaluation of effect on Escherichia coli and Staphylococcus aureus of radiolabeled ampicillin‐loaded graphene oxide nanoflakes. (10th February 2018)

• Record Type: Journal Article
• Authors: Yurt, Fatma; Ersöz, Onur Alp; Harputlu, Ersan; Ocakoglu, Kasim
• Is Part Of: Chemical biology & drug design. Volume 91:Number 6(2018)
• Page Start: 1094
• Page End: 1100
• Publication Date: 2018-02-10
• Subjects: ampicillin -- drug delivery -- graphene oxide -- in vitro uptake -- radiolabeled; Drugs -- Design -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; 615.19005
• DOI: 10.1111/cbdd.13171
• Languages: English
• ISSNs: 1747-0277

Abstract

Abstract : Ampicillin is a one of effective antibiotics against Gram‐positive and Gram‐negative bacteria. This study aimed to label ampicillin‐loaded graphene oxide nanoflake (AMP‐GO) with 99m Tc and evaluate of its in vitro binding to Staphylococcus aureus and Escherichia coli . Firstly, ampicillin was loaded into graphene oxide nanoflake prepared. AMP‐GO was characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) techniques, and the amount of loaded ampicillin onto GO was determined by UV‐Vis absorption spectroscopy. AMP and AMP‐GO were labeled with 99m Tc using stannous chloride reducing agent. Labeling efficiency of 99m Tc‐AMP‐GO was found to be 97.66 ± 2.06%. 99m Tc‐AMP‐GO has higher binding efficiencies to both S. aureus and E. coli than 99m Tc‐AMP. 99m Tc‐AMP‐GO could be promising candidate as agent infection nuclear imaging. Furthermore, in vivo studies of 99m Tc‐AMP‐GO with infected rats are planned to be performed. Abstract : Ampicillin‐Graphene oxide (AMP‐GO) nanoparticles by evaluating their in vitro binding in Staphylococcus aureus and Escherichia coli for infection nuclear imaging.