Lipopolysaccharide and cAMP modify placental calcitriol biosynthesis reducing antimicrobial peptides gene expression. (1st March 2018)

• Record Type: Journal Article
• Authors: Olmos‐Ortiz, Andrea; García‐Quiroz, Janice; Avila, Euclides; Caldiño‐Soto, Felipe; Halhali, Ali; Larrea, Fernando; Díaz, Lorenza
• Is Part Of: American journal of reproductive immunology. Volume 79:Number 6(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-03-01
• Subjects: antimicrobial peptides -- calcitriol -- cyclic adenosine monophosphate -- lipopolysaccharide -- placenta; Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12841
• Languages: English
• ISSNs: 1046-7408

Abstract

Abstract : Problem: Calcitriol, the hormonal form of vitamin D3 (VD), stimulates placental antimicrobial peptides expression; nonetheless, the regulation of calcitriol biosynthesis in the presence of bacterial products and its consequence on placental innate immunity have scarcely been addressed. Method of study: We investigated how some bacterial products modify placental VD metabolism and its ability to induce antimicrobial peptides gene expression. Results: Cultured human trophoblasts biosynthesized calcitriol only in the presence of its precursor calcidiol, a process that was inhibited by cyclic‐AMP but stimulated by lipopolysaccharide (LPS). Intracrine calcitriol upregulated cathelicidin, S100A9, and β‐defensins (HBDs) gene expression, while LPS further stimulated HBD2 and S100A9 . Unexpectedly, LPS significantly repressed cathelicidin basal mRNA levels and drastically diminished calcidiol ability to induce it. Meanwhile, cyclic‐AMP, which is used by many microbes to avoid host defenses, suppressed calcitriol biosynthesis, resulting in significant inhibition of most VD‐dependent microbicidal peptides gene expression. Conclusion: While LPS stimulated calcitriol biosynthesis, cyclic‐AMP inhibited it. LPS downregulated cathelicidin mRNA expression, whereas cyclic‐AMP antagonized VD‐dependent‐upregulation of most antimicrobial peptides. These findings reveal LPS and cyclic‐AMP involvement in dampening placental innate immunity, highlighting the importance of cyclic‐AMP in the context of placental infection and suggesting its participation to facilitate bacterial survival. Abstract : The bioconversion of calcidiol into calcitriol by human syncytiotrophoblasts is potentiated by LPS but antagonized by cAMP. LPS strongly represses cathelicidin gene expression, but stimulates S100A9 and HBD2. cAMP antagonizes the VD‐dependent stimulation of all antimicrobial peptides.