Development and characterization of 18 polymorphic SSR markers for Barthea barthei (Melastomataceae)1. (11th April 2017)

• Record Type: Journal Article
• Authors: Huang, Guilian; Liu, Haijun; Sun, Hongbin; Liu, Ying; Zhou, Renchao; Liao, Wenbo; Fan, Qiang
• Is Part Of: Applications in plant sciences. Volume 5:Number 4(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-04-11
• Subjects: Barthea barthei -- disjunct distribution -- genetic diversity -- Melastomataceae -- simple sequence repeat (SSR) marker; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.3732/apps.1600149
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the study: To examine population differentiation, simple sequence repeat (SSR) markers were developed in Barthea barthei, a shrub with a disjunct distribution in the southern mainland of China and Taiwan. Methods and Results: We used Illumina HiSeq technology to sequence a genomic library for SSR identification. Twenty‐seven SSR loci were developed, of which 18 SSR loci were polymorphic in three populations composed of two varieties of B. barthei . At the population level, the number of alleles ranged from one to seven, and the observed and expected heterozygosity varied from 0 to 0.850 and from 0 to 0.809, respectively. Higher genetic differentiation between the two populations of B. barthei var. barthei ( F ST = 0.474) was observed relative to the two varieties ( F ST = 0.387 and 0.418, respectively). Conclusions: These polymorphic SSR markers may be useful for understanding phytogeographic history of B. barthei . Lower genetic differentiation between the two varieties than between the two populations of B. barthei var. barthei suggests that the taxonomic treatment may not hold.